酪蛋白糖巨肽对大鼠肠黏膜屏障的保护作用-食品科学.pdfVIP

254 2013, Vol.34, No.07 食品科学 ※营养卫生 酪蛋白糖巨肽对大鼠肠黏膜屏障的保护作用 * 王金凤，陈庆森 ，闫亚丽 (天津市食品生物技术重点实验室，天津商业大学生物技术与食品科学学院，天津 300 134) 摘  要：目的：探讨不同剂量酪蛋白糖巨肽(CGMP)干预结直肠癌大鼠过程中，肠三叶因子(ITF/TFF3)和血管内皮生 2 长因子(VEGF)表达的变化情况，从而推断CGMP对大鼠肠黏膜屏障 种关键因子表达的影响。方法：40只Wistar雄 性大鼠随机分为正常对照组、模型组、低剂量CGMP组、中剂量CGMP组和高剂量CGMP组。除正常对照组外，对 每只大鼠每周腹腔注射1,2-二甲基肼二盐酸盐(DMH) ，剂量为30mg/kg ，同时，3个剂量CGMP组注射DMH 的同时每 天灌胃CGMP ，剂量分别为10、50、100mg/(kg•d) 。8周后，处死大鼠，取结肠组织，采用免疫组化方法检测TFF3和 VEGF 的表达情况。结果：HE染色结果表明CGMP可以改善大鼠肠黏膜的损伤；免疫组化结果证实了CGMP可以显 著降低大鼠肠黏膜VEGF 的表达，而对TFF3的表达没有影响。结论：CGMP具有修复大鼠肠黏膜屏障损伤的功能。 关键词：酪蛋白糖巨肽(CGMP) ；结直肠癌；肠黏膜屏障；肠三叶因子；血管内皮生长因子 Protective Role of CGMP in Intestinal Mucosal Barrier Function in Rats * WANG Jin-feng，CHEN Qing-sen ，YAN Ya-li (Tianj in Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianj in University of Commerce, Tianj in 300 134, China) Abstract ：Objective: To explore the effect of caseino glycomacropeptide(CGMP) intervention on the expression of trefoil factor and vascular endothelial growth factor in the colon and deduce the effect of CGMP on key factors of intestinal mucosal barrier function in rats with colorectal cancer. Methods: A total of 40 Wistar rats were randomly divided into five groups including control group, model group, low-dose CGMP group, middle-dose CGMP group and high-dose CGMP group. Except the control group, all the rats were intraperitoneally injected with dimethyl hydrazine at a dose of 30 mg/kg. At the same time, the rats from CGMP groups were injected with