外周血CXCL9和CXCR3在大鼠肾移植急性排斥-第三军医大学学报.docVIP

外周血CXCL9和CXCR3在大鼠肾移植急性排斥反应的表达及意义 李家兵，何云锋，夏 康，吴小侯（重庆医科大学附属第一医院泌尿外科，重庆400016） 摘 要：目的 探讨大鼠肾移植手术前后趋化因子CXCL9及其受体CXCR3 mRNA的动态变化在术后急性排斥早期诊断的作用。方法 以BN和LEW大鼠作为供体，LEW大鼠作为受体，建立大鼠同种异体肾移植模型。采用ELISA法和逆转录聚合酶链反应（RT-PCR）分别动态检测大鼠术前后血清CXCL9和外周血淋巴细胞CXCR3 mRNA的变化。另外同时检测血清肌酐水平。结果 实验组大鼠血清CXCL9，外周血淋巴细胞CXCR3 mRNA及血清肌酐表达均明显升高，与对照组比较差异有统计学意义（P＜0.01）。术前血清CXCL9和外周血淋巴细胞CXCR3 mRNA在实验组与对照组比较无统计学差异（P＞0.05），术后第1，3，5，7天实验组均高于对照组，差异有统计学意义（P＜0.05）。术前及术后第1天血清肌酐在实验组和对照组比较无统计学差异,术后第3，5，7天实验组均高于对照组，差异有统计学意义（P＜0.05）。结论 血清趋化因子CXCL9和外周血淋巴细胞CXCR3 mRNA的表达与排斥反应密切相关，可以作为早期诊断急性排斥反应的辅助敏感指标。 关键词：肾移植；急性排斥反应；趋化因子；趋化因子受体 中图分类号：R669.2 文献标识码: A The expression ands significance of CXCL9 and CXCR3 in peripheral blood in acute rat renal allografts rejection LI Jia-bing，HE Yun-feng，XIA Kang，WU Xiao-hou（department of urology,the first affiliated hospital of chongqing medical university,chongqing 400016,China） Abstract: Objective To evaluate the diagnostic role of chemokine CXCL9 and chemokine receptor CXCR3 mRNA in predicting the early stage of acute rejecton afer renal transplantation in rats. Methods: Renal allogeneil graft was performed using Brown Norway or Lewis rats as donors and Lewis rats as recipients. CXCL9 in serum was measured by ELISA and CXCR3 mRNA in peripheral blood lymphocytes by RT-PCR at the time points of 1day before operation and 1day,3days,5days,7days after operation. Serum creatinine(Cr) was tested simultaneously.Results The level of CXCL9 ,CXCR3 mRNA and Cr in allograft group were significantly higher than that in isograft group（P＜0.01）.There was no significant difference of CXCL9 and CXCR3 mRNA level in both allograft and isograft groups before operation, and was significantly higher on 1day,3days,5days and 7days after operation in allograft group than that in isograft group（P＜0.05） . There was no difference of Cr level in allograft and isograft groups before and 1day after operation , which was significantly higher on 3days,5days and 7days in allograft group than that in isograft group（P＜