Alexander staining(一种花粉活性染色法).pdfVIP

International Journal of Plant Biology 2010; volume 1:e13 A simplified method cein diacetate that was initially developed to for differential staining examine the viability of mammalian cells.5,6 Correspondence: Changbin Chen, Department The uses of fluorescent dyes, however, of Horticultural Science, University of Minnesota of aborted and non-aborted require special equipment, such as fluores- 380 Alderman Hall, 1970 Folwell Avenue, St. Paul, po en grains cence microscopes, which limit the applica- MN 55108, USA. E-mail: chenx481@umn.edu tion for field studies and most agricultural 1 2 extension stations that do not have the avail- Key words: pollen staining, Alexander’s stain, Ross Peterson, Janet P Slovin, chloral hydrate, phenol. 1 able equipment. Changbin Chen A major improvement in differential pollen 1 Department of Horticultural Science, staining was reported by M.P. Alexander in Contributions: RP did most of experiments and contributed to the writing; JPS did further test University of Minnesota, MN, USA; 1969. Alexander’s stain colors aborted pollen and contributed to edit the manuscript; CC initi- 2Genetic Improvement of Fruits and grains from