《女贞子中总黄酮含量的测定》-毕业设计（论文）.doc

PAGE 毕 业 设 计（论文） 题 目：女贞子中总黄酮含量的测定 你的论文题目是：女贞子总黄酮提取工艺研究 学 院： 化学与材料工程学院 专 业： 生物工程 学 号： 0740820120 学生姓名： 付志雄 指导教师： 徐玲花 年 月 日 黄石理工学院 毕业设计（论文） PAGE 26 摘 要 本文以女贞子为材料，研究了索式提取法提取女贞子中总黄酮的工艺条件，并通过颜色反应法，利用可见分光光度法来初步测定女贞子中的总黄酮的含量。 首先，以芦丁为标准品，通过铝盐显色法用紫外分光光度计扫描400nm~600nm波长范围，确定女贞子中提取总黄酮的最佳测定波长。然后利用可见分光光度法，得出女贞子总黄酮含量的标准曲线，标准曲线的回归方程为C=0.1288A+0.0006，其相关系数r=0.9994。在研究提取女贞子中总黄酮的工艺过程中，对女贞子不同部位中的总黄酮含量进行了对比，得出女贞子果皮中黄酮含量最高。并利用可见分光光度法以乙醇为提取剂通过单因素实验确定女贞子中总黄酮的最佳提取条件，并用正交实验确定各因素(温度、乙醇浓度、时间、固液比)的影响大小，为女贞子中总黄酮的开发提取提供依据。确定的最佳提取工艺条件为：乙醇浓度80%，料液比1:40，提取温度90℃，提取时间2h，测得黄酮的含量为11.827。 关键字：女贞子；总黄酮；提取；测定 ABSTRACT In this paper, the technological conditions of extraction flavonoids from Ligustrum lucidum were studied. Then flavonoids were proved to be flavonl by color reaction and UV spectrum. Firstly, through the aluminum salt color method using spectrophotometry scanning 400~600nm wavelength range, for ascertain the best determination wavelength of flavonoids. Then using spectrophotometry plotted the standard curve of flavones. The regression equation was C=0.1288A+0.0006 and the correlation coefficient R was 0.9994. In the study of extracting total flavonoids containing processes from Ligustrum lucidum, different parts of Ligustrum lucidum containing flavonoids content were compared, the result is that the rind contains highest content of flavonoid. Visible spectrophotometry using ethanol as the best extraction agent determined through single factor experiment of total flavonoids in Ligustrum lucidum, find out the best conditions for the reference substance. Rutin use as standard to draw curve map, and orthogonal experiment to determine the factors (temperature, ethanol concentration , time, solid-liquid ratio) in size, for total flavonoids in Ligustrum lucidum extract provide the basis for the development. Through this experiment we found out the best condition of extracting the total flavo