1.流式细胞仪原理与在cd4检测中应用.pptVIP

百分率计算：阳性群体所占的百分含量（%）； 例如：CD3：70%淋巴细胞，CD19：%10淋巴细胞 绝对计数：阳性群体的绝对数量（个细胞/微升体积）； 例如：AIDS患者CD4：50个细胞/ul全血 * * This procedure applies to the clinical laboratory environment with the BD FACSCount flow cytometer for the purpose of CD4 lymphocyte enumeration using whole blood specimens. We will use this procedure during the laboratory exercise Prime the system Disconnect both sheath tanlk and waste tank by removing quik-disconnects and sensors for both tanks. Empy the waste, add 200 ml hypochlorite Fill the sheath tank with sheath fluid up to one inch from the top. Replace reserviors, fluidic tubing