棉花轮纹斑病接种方法的优化.pdfVIP

棉 花 学报 Cotton Science 20 18袁30 6冤院486―491 棉花轮纹斑病接种方法的优化 1,2 1 1 1 1 1 2 1* 王婧 翟伟卜 孟菁 高环 史金艳 张文蔚 韩榕 齐放军 1. 国农业科学院植物保护研究所/ 植物病虫害生物学国家重点实验室袁 北京100193 曰2. 山西师范大学生命科学学院袁山西临汾041004 冤 摘要院揖 目的铱 棉花轮纹斑病是严重危害棉花生产的叶部病害遥 本文旨在优化棉花轮纹斑病菌链格孢 冤的接种方法遥 揖方法铱利用链格孢孢子悬浮液袁比较研究了涂菌+ 套袋保湿接种尧涂菌+ 喷 雾保湿接种和喷菌+ 喷雾保湿接种的效果遥揖结果铱采用涂菌+ 喷雾保湿接种袁接种的棉花叶片易于发病袁且能 有效区分抗病与感病品种袁但涂菌法伤叶不均匀袁叶片发病随机性大袁存在一定的不均匀性曰喷菌+ 喷雾保湿 接种袁接种的棉花叶片发病周期相对较长袁病程较为缓慢袁但和另外2 种接种方法相比袁发病的一致性好遥 揖结 论铱喷菌+ 喷雾保湿接种是1 种更适合棉花链格孢抗性研究的接种方法遥 关键词院棉花曰轮纹斑病曰链格孢曰接种方法曰方法优化 中图分类号院S435.621 文献标志码院A 文章编号院1002-7807(2018)06-0486-06 10.11963/ 1002-7807.wj qfj 1,2 1 1 1 1 1 2 1* Wang Jing , Zhai Weibo , Meng Jing , Gao Huan , Shi Jinyan , Zhang Wenwei , Han Rong , Qi Fangjun (1. 100193 2. 041004 ) [Objective] Cotton Alternaria leaf spot disease causes serious damage during cotton production. The aim of this study was the optimization of an inoculation method for cotton Alternaria leaf spot. [Method] An spore suspension was used as the inoculum. Three inoculation methods were tested and compared: smearing with spores plus retaining moisture using a sealed plastic bag; smearing with spores plus retaining moisture by intermittently spraying with water; and spraying spores plus retaining moisture by intermittently spraying with water. [Result] Inoculated cotton leaves were most readily infect- ed by smearing with spores plus intermittently spraying with water. This inocula