34种秋海棠基因组大小比较与分析.docxVIP

34种秋海棠基因组大小比较与分析 杜文文1，王祥宁1，段 青1，贾文杰1，马璐琳1，崔光芬1＊，王继华1＊ (1云南省农业科学院花卉研究所/国家观赏园艺工程技术研究中心， 昆明 650205) 摘要：以34种野生秋海棠（包括4个变种）为试材，水稻（Oryza sativa L. ssp. japonica）为外标，采用流式细胞法测定其基因组大小，比较不同种、组之间基因组大小的差异，并分析与染色体数的相关性。结果表明：34种秋海棠基因组1C值在0.292～2.554 pg之间，最大值约为最小值的9倍，平均基因组大小为0.863 pg，最小的为盾叶秋海棠（B. 拉丁学名第一次出现需补全属名peltatifolia），最大的为水鸭脚秋海棠（B. fomosana）。中国原产的30种秋海棠平均基因组1C值C值还是1C值？需核实全文(1C=0.925 pg)较南美洲原产的4种（1C=0.398 pg）大，台湾原产的3种1C值均比大陆原产的27种大。中国原产秋海棠不同组间基因组的大小存在差异，同一组内基因组大小亦不相同，本研究所测材料以四室组的基因组最大，为1.285 pg，组内变化近3.2倍；秋海棠组和二室组次之，分别为0.895 pg和0.888 pg，组内变化近6.4、6.8倍；侧膜胎座组基因组最小，为0.721 pg，组内变化约1.2倍。相关性分析表明秋海棠基因组大小与染色体数无显著相关性。本结果可为秋海棠遗传多样性分析及基因组学研究提供一定的基础数据。 拉丁学名第一次出现需补全属名 C值还是1C值？需核实全文 关键词：秋海棠；基因组大小；染色体数 Genome Size Comparison in 34 Begonia Species DU Wen-wen1, WANG Xiang-ning1 , DUAN Qing1, JIA Wen-jie1, MA Lu-lin1, CUI Guang-fen1, WANG Ji-hua1 （1Flower Research Institute of Yunnan Academy of Agricultural Sciences/National Ornamental Horticulture Engineering Technology Research Center, Kunming 650205） Abstract: Genome sizes of a Begonia collection comprising 34 species (including four varieties) were screened by flow cytometry, using Oryza sativa L. ssp. Japonica as an external reference, the differences in genome size between different speices and sections were compared, correlation between genome size and chromosome number was analysed. The results showed that 1C values varied between 0.292 pg and 2.554 pg, a 9-fold difference was found among genotypes with genome size, the mean genome size of 34 Begonia species was 0.863 pg, the genome of B. peltatifolia was smallest, the genome of B. fomosana origined from Taiwan was largest. The mean genome size of 30 Begonia species in China origin (1C=0.925 pg) was lager than 4 Begonia species’ in South American origin (1C=0.398 pg), genome sizes of 3 Begonia species in Taiwan origin were all larger than Chinese origin Begonia species’. There were significant differences in the genome size between different sections of Chinese Begonias, and in the same