北京棒杆菌Corynebacteriumpekinense天冬氨酸激酶P184M酶动力.pdf

8 5 Vol. 8 No. 5 2017 5 Journal of Food Safety and Quality May. , 2017 Corynebacterium pekinense P184M * 王锐楠, 张芷睿, 方 丽, 闵伟红 , 刘春雷 (/, 130118) 摘 要: 目的 (aspartate kinase, AK)184 (proline, P) (DL-methionine, M)方法 AK , Pro184 , , P184M结果 : P184M AK V WT(wide type)5.06 ; n 0.19, WT max , Km(mol/L); AK pH 6.5, pH 7.0; 28 ℃ , WT 30 ℃ , ; WT 2.1 h 1.2 h; Thr+MetLys+MetThr+Lys+Met AK 结论 P184M , 关键词: ; ; ; Enzyme kinetics analysis and enzymatic properties of aspartate kinase P184M from Corynebacterium pekinense * WANG Rui-Nan, ZHANG Zhi-Rui, FANG Li, MIN Wei-Hong , LIU Chun-Lei (National Engineering Laboratory on Wheat and Corn Further Processing , College of Food Science and Engineering, Jilin Agricultural University , Changchun 130118, China) ABSTRACT: Objective To investigate the effects of site 184 proline (P) of aspartate kinases (AK) mutationed to DL-methionine (M) on enzyme kinetics and enzymatic properties. Methods Mutant P184M was successfully constructed by saturation site-directed mutagenesis of highly conserved amino acid