土壤微生物多样性研究的新方法.pdfVIP

41 1 Vol41, No1 2004 1 ACTA PEDOLOGICA SINICA Jan. , 2004 马 万 里 ( , , 010022) Josquin Ti its Mark Adams (Forest Science Center, University of Mel oume, Creswick, VIC3363,Australia) , , , DNA (TRFLP) , DNA , PCR TRFLP TRFLP ; 16S DNA; DNA ITS; TRFLP Q93996 A , ( ( DNA) , , ) ( ) , PCR , [ 1~ 3] , : ARDRA ( Amplified ri osomal DNA restriction analysis) , SSCP ( Single Strand Confor , , mation Polymorphism) , RFLP( Restriction fragment length [4~ 7] : polymorphism) , Sequencing , DGGE ( Denaturing gradient , gel electrophoresis) TRFLP (Terminal restriction frag , , ment length polymorphism) , , , [ 7, 8] , , [ 3~ 7] , , DNADNA , , , [ 1,5, 7~ 10] , DNA , , DNA , DNA [ 1, 10, 11] , , , Pine Eu , DNA , calyp