桑叶中添加三聚氰胺对家蚕生长及代谢的影响.pdfVIP

Chinese Journal of Applied Entomology 2018, 55(2): 243250. DOI: 10.7679/j.issn.20951353.2018.034 桑叶中添加三聚氰胺对家蚕生长及代谢的影响* 贾漫丽1, 2** 李 娜1, 2 谢 岩1, 2 黄 露1, 2 杨贵明1, 2 李季生1, 2*** 1. 067000 2. 067000 摘 要 【目的】 MelamineMEL 【方法】 5 600 4 3 50 3 101520 mg/kg72 h 【结果】 20 mg/kg P0.05 【结论】 关键词 Effects of adding melamine to mulberry leaves on the growth and digestive metabolism of silkworms JIA Man-Li1, 2** LI Na 1, 2 XIE Yan 1, 2 HUANG Lu 1, 2 YANG Gui-Ming 1, 2 LI Ji-Sheng 1, 2*** (1. Hebei Universities RD Centre for Sericulture and Specialty Enabling Technologies, Chengde 067000, China; 2. Institute of Sericulture, Chengde Medical University, Chengde 067000, China) Abstract [Objectives] To investigate the effects of adding melamine (MEL) to mulberry leaves on the growth and serum biochemical indices of silkworms, and thereby reveal the metabolic pathways by which ingestion of a nonlethal dose of MEL produces toxic effects in this species. [Methods] 600 newly exuviated 5th instar larvae of approximately the same size were randomly divided into four groups, each group having 3 replicates of 50 silkworms (25 males and 25 females). The control group were fed fresh mulberry leaves whereas the three treatment groups were fed fresh mulberry leaves to which one of three nonlethal doses (10, 15 and 20 mg/kg) of MEL had been added. Monitoring of phenotypic and serum biochemical indices began 5-days after silkworms had begun feeding. [Results] The addition of MEL slowed the relative growth rate of larvae, significantly so (P0.05) in the 20 mg/kg MEL treatment group. Glycometabolism and haemolymp