移植部位对小鼠睾丸移植效果的影响.docVIP

移植部位对小鼠睾丸移植效果的影响 张艺宝1，李 淼2，郁丽丽3，强苏静3，刘丽均3，徐 平2,3，芮 荣1 （1南京农业大学动物医学院，南京210095：2.中国科学院上海生命科学研究院，上海201615；3..上海斯莱克实验动物有限责任公司，上海201615） 【摘要】 目的 比较不同的移植部位对睾丸移植效果的影响，以探索建立疾病模型小鼠安全保存的新方法。方法 按移植部位的不同分为3组：背部皮下组，睾丸白膜内组和肾包膜下组。移植后处死受体鼠回收移植物。测试和分析受体精囊的重量，移植物存活率，回收物增重的倍数和生殖细胞的分化情况。 结果 不同实验组移植睾丸的生殖细胞分化差异显著。睾丸白膜内组的分化情况最好与假移植对照组相似；背部皮下移植组睾丸的分化率为29.2%；肾包膜下组未见分化。结论 睾丸白膜内移植最利于精子的发生；背部皮下移植是睾丸移植的次优选择；肾包膜下睾丸移植不适合用于小鼠雄性生殖器官的安全保存。 【关键词】小鼠，睾丸移植，移植部位，生殖细胞分化 The Effect of Different Position for Testicular Transplantation in Mouse ZHANG Yi-bao1, LI Miao2, YU Li-li3, QIANG Su-jing3, LIU Li-jun3, XU Ping2,3, RUI Rong1 (1. College of Veterinary Medicine, Nanjing Agriculture University, Nanjing 210095: 2.Shanghai Institutes for Biological Sciences, Chinese Academy of Science，Shanghai 201615；3.Shanghai SLAC Laboratory Animal Ltd. Co.，Shanghai 201615) 【Abstract】 Objective To compare the testicular transplant efficiency of different position for establishing a new preservation techniques in disease mouse models. Method Dorsal subcutaneously transplant group, testicular membrane transplant group and subrenal capsule transplant group were used for evaluating the effect of transplant position. The transplanted testes were collected after experiment, testing and analysis their weight, transplant survival, weight gain, and germ cell differentiation. Results There are significant difference of the testicular germ cell differentiation in different transplantation groups . The germ cell differentiation of testicular membrane transplant group are same as control group; the differentiation rate of germ cell was 29.2% in dorsal subcutaneously transplant group; and there was no differentiation in subrenal capsule transplant group. Conclusions The testicular membrane is a favorable position for testicular transplantation， and dorsal subcutaneously transplantation is an alternative choice. Subrenal capsule transplantation are not appropriate for preservation of male reproductive organs in mice. 【Key words