卵细胞促成熟因子.pptVIP

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2019-12-23 发布于湖北
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卵细胞促成熟因子.ppt

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EXPERIMENTAL PATHWAYS 1.What’s the Cyclins? 2.What’ the relationship between cyclins and MPF? What’s the cyclins? 一、 MPF 卵细胞促成熟因子，matuation-promoting factor；细胞促分裂因子，mitosis-promoting factor； M期促进因子，M-phase-promoting factor）。二、Cyclins 细胞周期素周期蛋白激酶 CDK ( cyclin dependent kinase), P619 Kinetics of Xenopus oocyte activation by progesterone and cyclinA mRNA What’s the relationship between cycling and MPF? cyclinA is an activator of MPF MPF=CDC2+Cyclin B =a complex Cyclin B的降解途径 P34cdc2 The yeast protein was called p34cdc2 or simply cdc2. The first evidence of a link between cdc2 and MPF came as the result of a collaboration between yeast and amphibian research group Recall from fission yeast were shown to react specifically with the 32- and 45-kDa protein Antibodies formed against cdc2 from fission yeast were shown to react specifically with the 32-kDa component of MPF isolated from Xenpus eggs. This component of MPF is a homologue of the 34-kDa yeast kinase and, the machinery controlling the cell cycle in yeast and vertebrates contains evolutionarily conserved components. 2001年10月8日, L.?Hartwell、P. Nurse、T.?Hunt 因对细胞周期调控机理的研究而荣获诺贝尔生理医学奖。 * * yeast: Cln1,Cln2,Clin3,Clb1-Clb6 Vertabrate: A1-2、B1-3 、C、 D1- 3、E1-2、E、F、G、H等 Every cyclins has a 100 amides keep sequence, which called cyclin-frame.It can interpose cyclin combine with CDK(cycling dependent kinase), different cyclins will recognize different CDK to make a cycling-complex. M- Different cycling expression in different time and combine with different CDK to accommodate different CDKase activition. 注射实验表明： 1.孕酮诱导卵母细胞成熟； 2.成熟卵细胞质中，含有卵母细胞成熟的因子，称做MPF GVBD% MPF