ox-LDL损伤的内皮细胞中microRNA表达变化.docVIP

MicroRNAs expression in ox-LDL treated HUVECs:MiR-365modulates apoptosis and Bcl-2expression Bing Qin a , Bo Xiao a , Desheng Liang b , Jian Xia a , Ye Li a , Huan Yang a , ? a Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China b State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410078, PR China a r t i c l e i n f o Article history: Received 18May 2011 Available online 26May 2011Keywords:MicroRNA Ox-LDL HUVECs Apoptosis Atherosclerosis a b s t r a c t Endothelial cells (ECsapoptosis induced by oxidized low-density lipoprotein (ox-LDLis thought to play a critical role in atherosclerosis. MicroRNAs (miRNAsare a class of noncoding RNAs that posttranscription-ally regulate the expression of genes involved in diverse cell functions, including differentiation, growth, proliferation, and apoptosis. However, whether miRNAs are associated with ox-LDL induced apoptosis and their effect on ECs is still unknown. Therefore, this study evaluated potential miRNAs and their involve-ment in ECs apoptosis in response to ox-LDL stimulation. Microarray and qRT-PCR analysis performed on human umbilical vein endothelial cells (HUVECsexposed to ox-LDL identi?ed 15differentially expressed (4up-and 11down-regulated miRNAs. Web-based query tools were utilized to predict the target genes of the differentially expressed miRNAs, and the potential target genes were classi?ed into different function categories with the gene ontology (GOterm and KEGG pathway annotation. In particular, bioinformatics analysis suggested that anti-apoptotic protein B-cell CLL/lymphoma2(Bcl-2is a target gene of miR-365, an apoptomir up-regulated by ox-LDL stimulation in HUVECs. We further showed that transfection of miR-365inhibitor partly restored Bcl-2expression at both mRNA and protein levels, leading to a reduction of ox-LDL-mediated apoptosis in HUVECs. Taken together, our ?ndings indicate that miRNAs participate in ox-LDL-med