哮喘气道炎症粘附机制的实验研究.docVIP

???? 哮喘气道炎症粘附机制的实验研究 ???? ????　【摘要】　目的　评价细胞间粘附分子1（ICAM-1）、P选择素在哮喘气道炎症粘附机制中的作用，进一步阐明哮喘的发病机理。方法　用酶联免疫吸附试验、肺组织免疫组化检查和呼吸生理学方法系统观察正常组和哮喘组豚鼠各项指标。结果　(1)哮喘组豚鼠肺潮气量、动态肺顺应性(Cdyn)和肺气道阻力与对照组比较差异有显著性（P0.01及P0.05)。(2)哮喘组豚鼠血浆和肺泡灌洗液（BALF）可溶性ICAM-1(sICAM-1)、可溶性P选择素、血清和BALF嗜酸粒细胞阳离子蛋白(ECP)与对照组比较，差异有显著性(P0.01)；BALF中白细胞介素8(IL-8)与对照组比较差异也有显著性(P0.01)。(3)哮喘组豚鼠肺组织(气道上皮和血管内皮)ICAM-1和IL-8表达与对照组比较，差异有显著性(P0.01)。结论　ICAM-1、P选择素、IL-8、ECP参与介导了哮喘气道炎症的粘附过程。　　【关键词】　哮喘　　白细胞介素8　　嗜酸粒细胞阳离子蛋白Experimental study on adhesion mechanism of airway inflammation in the pathogenesis of bronchial asthma　　LI Yin, YE Yaoqin. Department of Respiratory Medicine, Shanghai NO.455 Hospital, Shanghai 200052　　【Abstract】　Objective　To investigate the adhesive mechanism of airway inflammation in the pathogenesis of asthma. Methods　The guniea pigs were divided into 2 groups,the asthmatic animal group and the normal animal group.The following tests were performed: (a) The values of sICAM-1,sP-selectin,IL-8 and ECP both in plasma (or serum) and BALF were measured by ELISA.(b) The expressions of ICAM-1 and IL-8 on the bronchial epithelial cells and vascular endothelial cells were measured by immuno-histochemistry staining.(c) The lung functions(VT,Cdyn,RL)were tested. Results　(a) The values of sICAM-1,sP-selectin and ECP were elevated both in the plasma and BALF level of asthmatic group (P0.01). Additionally,IL-8 of BALF level of asthmatic group was elevated (P0.01), while IL-8 of serum level was lower than that of normal group（P0.01). (b)The expressions of ICAM-1,IL-8 on the bronchial epithelial and vascular endothelial cells in asthmatic animals were up-regulated (P0.01). (c) The lung functions of the asthmatic animals were impaired (VT，Cdyn P0.01；RL P0.05). Conclusions　ALL the results above suggests that ICAM-1,IL-8,P-selectin and ECP are involved in the inflammative adhesive mechanism of asthma.　　【Key words】　Asthma　　Interleukin-8　　Eosinophilic cationic protein　　哮喘是一种由多种细胞特别是肥大细胞,嗜酸粒细胞及淋巴细胞参与的慢性气道炎症性疾病。哮喘发病机制复杂，尽管目前已了解细胞