川芎嗪对猪冠状动脉平滑肌细胞大电导钙激活钾通道的作用.docVIP

研究论文 Received 2005-06-20 Accepted 2005-08-29 This work was supported by the National Natural Science Foundation of China (No.and the Natural Science Foundation of Educational Department of Sichuan Province (No. 2003A058. * Corresponding author. Tel: +86-830-3160619; E-mail: wyangyan@ 川芎嗪对猪冠状动脉平滑肌细胞大电导钙激活钾通道的作用 杨艳艳,杨 艳*,曾晓荣,刘智飞,蔡 芳,李妙龄,周 文,裴 杰 泸州医学院心肌电生理学研究室,泸州646000 摘 要:本工作旨在研究川芎嗪对猪冠状动脉平滑肌细胞钾通道的作用,为阐明其扩张冠状动脉血管的机制提供实验依据。采用膜片钳细胞贴附式和内面向外式记录方式观察川芎嗪对猪冠状动脉平滑肌细胞大电导钙激活钾通道(large-conductance Ca 2+ -activated potassium channels, BK Ca channels的作用,分别用蛋白激酶A (protein kinase A, PKA抑制剂H-89和蛋白激酶G (protein kinase G, PKG抑制剂KT-5823处理细胞,再观察川芎嗪对BK Ca 通道作用的变化。结果表明在研究的0.73~8.07mmol/L 浓度范围,川芎嗪可以剂量依赖性地激活BK Ca 通道,使通道的开放概率从 (0.01±0.003 增加到(0.03±0.01~(1.21±0.18 (P 0.01,n =10,使通道平均关闭时间从(732.33±90.67 ms 降低到(359.67±41.30 ~ (2.96±0.52 ms (P 0.01,n =10。川芎嗪的这种激活作用在浴液游离钙离子浓度接近0 mmol/L 时也存在。PKA 的特异性抑制剂H-89 (3 μmol/L 和PKG 的特异性抑制剂KT-5823 (1 μmol/L对川芎嗪激活BK Ca 通道的作用无影响。以上结果提示:川芎嗪能直接激活冠状动脉平滑肌BK Ca 通道,这种作用可能是川芎嗪扩张冠状动脉血管的一种重要机制。关键词:川芎嗪;大电导钙激活钾通道;猪冠状动脉平滑肌细胞;膜片钳技术中图分类号:R331.3+6; R285.5 Effects of tetramethylpyrazine on large-conductance Ca 2+-activated potassium channels in porcine coronary artery smooth muscle cells YANG Yan-Yan, YANG Yan *, ZENG Xiao-Rong, LIU Zhi-Fei, CAI Fang, LI Miao-Ling, ZHOU Wen, PEI Jie Institute of Myocardial Electrophysiology, Luzhou Medical College, Luzhou 646000, China Abstract: The aim of the present study was to examine the effects of tetramethylpyrazine (TMP on large-conductance Ca 2+ -activated potassium channels (BK Ca channels in porcine coronary artery smooth muscle cells, in order to provide the experimental evidence for expounding the mechanism of TMP in dilating coronary artery. Cell-attached and inside-out single channel recording techniques were used to observe the effects of TMP on BK Ca channels as well as the effects after the cells were treated by protein kinase A (PKA inhibitor or protein kinase G (PKG inhibitor. In inside-out patch, TMP activated BK Ca