...Antigen DNA Vaccine against Cancer新型肿瘤趋化抗原基....pptVIP

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...Antigen DNA Vaccine against Cancer新型肿瘤趋化抗原基....ppt

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* Novel Chemotactic-Antigen DNA Vaccine against Cancer 新型肿瘤趋化抗原基因疫苗 Cancer Institute, Hospital Chinese Academy of Medical Sciences Peking Union Medical Collage Shuren Zhang 张叔人 Intratumoral SLC administration promotes Th1 cytokine and antiangiogenic chemokine release and a decline in immunosuppressive mediators Th1 cytokine : IFN-g, GM-CSF, IL-12, MIG, and IP-10 Immunosuppressive mediators: PGE2, IL-10, and TGF-b angiogenic factor: VEGF The Journal of Immunology, 2000, 164: 4558–4563. Steven M. Dubinett Regulation of Cytokines secretion by secondary lymphoid tissue chemokine (SLC) Chemotactic-Antigen DNA Vaccine (CADV) 趋化抗原基因疫苗 Ag Her2,TERT PSA,PAP CEA,HPV SLC Fc CMV Enzyme Cut Sites DC Th CTL T B CCR7 FcR CCR7 Gene transfer and expression Recruiting DC and T cell ; catching Ag by ligands; Ag presentation T and B cell activation, anti-Tumor Effects Effect Mechanisms of CADV Tumor CMV SLC Ag Fc EP MHC-II MHC-I Injection or Gene gun Antigen presentation by DCs through ligand-mediated internalization can be enhanced up to 10,000-fold over fluid-phase antigen pinocytosis Mouse Model of CADV mSLC Fc CMV Her2/P53 HPV16E7 E7 Mouse Model of CADV (pSLC-E7-Fc) RT-PCR and Western Blotting Analysis (A) RT-PCR detection of B16F10 cells transfected with various recombinant plasmids. DNA marker (M),Lane 1 (vector control), Lane 2 (SLC fragment), Lane 3 (Sig-E7 fusion fragment), Lane 4 (SLC-E7 fusion fragment),Lane 5 (Sig-Fc fusion fragment), Lane 6 (SLC-Fc fusion fragment), Lane 7 (Sig-E7-Fc fusion fragment), Lane 8 (SLC-E7-Fc fusion fragment). (B) Western blotting analysis of B16F10 transfectants. SLC-E7-Fc fusion protein (50kD), E7-Fc fusion protein (38kD),SLC-E7 fusion protein (22kD). Chemotaxis Assay by Chemotaxis Chamber pSLC-E7-Fc pcDNA HPV16-E7 as tumor antigen. E7+C3 tumor cell subcutaneous inoculated in C57BL/6 mice on