基因芯片生物信息学.pptxVIP

Microarray and Bioinformatics基因芯片的生物信息学 ;Aims for the Microarray Bioinformatics;Vocabulary-Review 回顾;Vocabulary-Review回顾;Microarray 基因芯片;Types of Microarrays 芯片的种类;Types of DNA Microarrays;History 历史;Microarrays are Popular 芯片技术的普及;What problems can it solve?基因芯片的应用;Features 特点;Differential Gene Expression基因表达差异;What is its pitfall 缺陷与不足?;Requriments;Market predict 市场预期;Principle 原理;Designing the Probes 探针的设计;Spotting Process 点样过程;点样针;;;Spot robot 点样仪;Affymetrix 基因芯片;表达差异检测;;Comparison of Probe Types两种探针比较;Homemade Tailored Cheaper????? Maximum 24,000 features per array Prone to variability;Process of manufacture a microarray芯片制备流程;;;Microarray Steps 基因芯片分析过程;Reading an array (cont.);Color Coding扫描结果;Noise 干扰;Other Image Processing Problems Spot Quality Problems;Two slides;Noise filtering 干扰过滤;Noise filtering 干扰过滤;Normalization 归一化;Normalization 归一化;Normalization 归一化;The origin of systematic differences系统误差的产生原因;DNA array Data AcquisitionDNA 芯片数据的获得;Introduction of Software----SAMSAM软件介绍;;;;;Handling Missing Data 丢失数据的操作;Clustering 聚类软件;Clustering = Group identification;Clustering Steps 聚类分析步骤;Cluster algorithm聚类算法;Hyerarchical Clustering Example;Hyerarchical Clustering Example; /cgi/content/full/95/25/14863;系统聚类法步骤;Hierarchical Clustering系统聚类法;Hierarchical Clustering 系统聚类;Hierarchical Clustering系统聚类;Interpreting the Results;k-means 聚类分析;;Recommended Texts;Affymetrix Michael Eisen Lab at LBL (hierarchical clustering software “Cluster” and “Tree View” (Windows)) / Stanford MicroArray Database (“Xcluster” (Linux)) /MicroArray/SMD/ Review of Currently Available Microarray Software /yr2001/apr/profile1_010430.html Microarray DB www.biologie.ens.fr/en/genetiqu/puces/bddeng.html;Eisen, M. B. et al., (1998).Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci U S A 95(25): 14863-8.Wen, X., et al., (1998). Large-scale temporal gene ex- pression mapping of central nervous system development. Proc Natl