原创力文档biologicalfunctionandmechanismoflongnoncodingrnasnuclearenrichedabundanttranscript1indevelopmentofcervicalcancertranslatedtitle长链非编码rnaneat1在宫颈癌发生中的生物学作用及其机制研究.pdfVIP
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Original Article
Biological Function and Mechanism of Long Noncoding RNAs
Nuclear‑Enriched Abundant Transcript 1 in Development of
Cervical Cancer
1,2 2 2 2 1 1
Hui‑Ling Wang , Shun‑Yu Hou , Hai‑Bo Li , Jian‑Ping Qiu , Le Bo , Cai‑Ping Mao
1Reproductive Medicine Center, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China
2Department of Gynaecology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215000, China
Abstract
Background: Accumulating documents have demonstrated that long noncoding RNAs (lncRNAs) play critical roles in tumorigenesis.
As an lncRNA, nuclear‑enriched abundant transcript 1 (NEAT1) has been identified to be involved in the progression of many types of
cancers. However, the biological function of NEAT1 in cervical cancer is not fully investigated. The aim of this study was to disclose the
specific biological function of lncRNA NEAT1 in cervical cancer progression.
Methods: Quantitative real‑time polymerase chain reaction (qRT‑PCR) was utilized to identify the expression of lncRNA NEAT1 in the
cervical cancer tissues and cell lines. All cervical cancer samples used in this study were collected from the Affiliated Suzhou Hospital of
Nanjing Medical University between September 2012 and September 2017. The correlation between NEAT1 expression and the overall
survival rate of cervical cancer patients was analyzed by Kaplan‑Meier analysis. The effects of NEAT1 knockdown or overexpression
on cell proliferation were tested by performing MTT assays and colony formation assays. Transwell assays were conducted to detect
the migratory ability of cervi
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