百蕊草愈伤组织离体培养条件的优化.docxVIP

百蕊草愈伤组织离体培养条件的优化 摘要：以百蕊草的幼嫩茎及叶片为外植体，探讨百蕊草外植体的最佳消毒时间、离体培养的培养基适宜配方、pH值以及培养温度。结果表明，最佳消毒时间为酒精消毒11 s后升汞消毒8 min；愈伤组织诱导增殖的最佳培养基为MS+1 mgL-1 6-BA+0.15 mgL-1 NAA+0.1 mgL-1 2，4-D；最适pH值为5.8～6.0；最佳培养温度为22～26 ℃。　　关键词：离体培养；愈伤组织；影响因素　　　　Abstract： Taken the tender branches and Leaves of Thesium Chinese Turcz as explants， the best method for sterilization of explants，best medium and culture conditions for callus induction were instigated on callus by using the orthogonal experiments. The results showed that the best disinfection for alcohol after was 11 s and mercuric chloride disinfection was 8 min， the best