EPA 4025 SCREENING FOR POLYCHLORINATED DIBENZODIOXINS AND POLYCHLORINATED-国外标准规范.pdfVIP

METHOD 4025 SCREENING FOR POLYCHLORINATED DIBENZODIOXINS AND POLYCHLORINATED DIBENZOFURANS (PCDD/Fs) BY IMMUNOASSAY 1.0 SCOPE AND APPLICATION 1.1 Method 4025 is a procedure for the analysis of PolyChlorinated DibenzoDioxins and PolyChlorinated DibenzoFurans (PCDD/Fs) in soil at 500 ppt (pg/g). Method 4025 uses an Enzyme Immunoassay (EIA) commercially available test kit containing a polyclonal antibody specific for PCDD/Fs. The EIA kit is designed for the screening of samples according to their toxic equivalent concentration (TEQ) by responding to the toxic PCDD/F congeners in approximate correlation with their toxic equivalency factors (TEFs). The test is capable of multiple congener recognition and preferentially targets congeners with high TEF values; i.e., those with the highest toxicity relative to 2,3,7,8- TetraChloroDibenzo-p-Dioxin (2,3,7,8-TCDD). The final measured EIA response is the sum of the individual congener responses. This response correlates with TEQ because the immunoassay cross-reaction profile for PCDD/Fs correlates with TEF values. See Table 2 for a detailed cross-reaction profile. 1.2 The testing product evaluated by EPA for this method employs a competitive enzyme immunoassay. It is important to note that this method differs from other 4000 series immunoassay methods due to the specific chemistry of the analyte. The chemistry of dioxin analysis is more difficult, as shown by the cost and turnaround time differences between dioxin/furan analysis (e.g. Method 8280/8290) and PCB analysis (e.g. Method 8082). Dioxins are 3 to 4 orders of magnitude less water soluble than most other 4000 series target analytes. Additionally, sensitivity targets can be orders of magnitude lower than for PCBs and other analytes, so the matrix concentration factors can be extreme an