Illumina测序的原理和应用【可修改】.pptxVIP

Illumina 测序的原理和应用 精选 Library Preparation 文库制备 Cluster Generation 簇生成测序流程 Sequencing 测序 Data Analysis 数据分析Illumina测序流程精选接头：与flow cell结合区Read1和Read2测序引物结合区Index 用来区分不同样本Adapter and Library接头和文库精选 Library Preparation 文库制备 Cluster Generation 簇生成测序流程 Sequencing 测序 Data Analysis 数据分析Illumina测序流程精选什么是 flow cell？Flow cell 即流动槽，如载玻片大小，有8个通道即8条lane。精选1. Single DNA libraries are hybridized to primer lawn and extended by polymerase精选2. Double-stranded molecule is denatured and original template is washed away精选3. New synthesized strand is covalently attached to flow cell surface to form a bridge and