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1 AS 5013.14.2—2009
Australian Standard®
A
S
Food microbiology
5
0
1
3
.
1
4 Method 14.2: General procedures and
.
2
2— techniques—Colony count—Membrane filtration
0
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9 method
PREFACE
This Standard was prepared by the Standards Australia Committee FT-024, Food Products
and Subcommittee FT-024-01, Food Microbiology (Constituted), to supersede
AS 1766.1.5—1991, Food microbiology , Method 1.5: General procedures and techniques—
Colony count—Membrane filtration method.
The objective of this revision is to update the references and to transfer the procedure to
AS 5013 series. It also incorporates minor technical variations on the apparatus used in the
test technique.
METHOD
1 SCOPE
This Standard sets out a method for estimating the number of colony-forming units (CFUs)
9 in suitable liquids using a membrane filtration technique.
0
0
2
g The method is applicable only to liquids that can be efficiently filtered without causing a
u
A
7 build-up on the filter.
2
n NOTE: The membrane filtration method is most suitable when the microorganisms are in low
o
A concentration, e.g. in rinse waters collected from cleaned and sanitized pipelines or tanks. The
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L
A method is suitable for microbiological assessment of water supplies to pro
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