实验研究医学论文--家猪急性心肌梗塞模型冠脉零流量压与存活心肌的相关性.doc

NF-κB抑制剂PDTC对小鼠急性心肌梗塞后Wnt信号通路的影响 作者：黄 莺1，马依彤1，杨毅宁1，刘 芬2，陈邦党2，李晓梅1 作者单位：国家自然科学基金项目;新疆维吾尔自治区重点实验室项目(XJDX0903) 1 新疆医科大学第一附属医院冠心病科，新疆 乌鲁木齐 830054;2 新疆心血管病研究所 　　【摘要】 目的：探讨核转录因子(NF)-κB抑制剂二硫代氨基甲酸吡咯烷(PDTC)对小鼠急性心肌梗塞(AMI)后Wnt信号通路的影响。方法：结扎3月龄雄性C57/BL小鼠左冠状动脉建立AMI模型，随机分成AMI+PDTC组(36只，术后每日腹腔注射PDTC 120mg/kg)，AMI组(36只，每日注射同上述剂量的生理盐水)。所有小鼠按心梗时间分3 d、7 d、14 d亚组，比较应用PDTC后NF-κB信号通道相关的p65，Wnt信号通路的Dishellved(dvl)-1 mRNA表达，糖原合成激酶(GSK)-3β总水平及磷酸化水平变化，β-连接蛋白(catenin)及缝隙通道连接蛋白(connexin)43表达。结果：与AMI 组相比，AMI+PDTC组的p65，dvl-1 mRNA表达显著降低(P均<0.05),p/t GSK-3β 3 d，7 d及14 d时均显著升高(P值均为0.000);βcatenin表达3 d时显著低于AMI组，7 d时显著高于AMI组(P值均为0.000);connexin 43表达显著降低(P=0.000)。结论：抑制NF-κB活性、信号通道后Wnt信号通路相关蛋白活性也发生变化，说明急性心肌梗塞后NF-κB信号通路与Wnt信号通路之间存在互通作用。 　　【关键词】 心肌梗塞;NF-κB;小鼠 　　Effects of NF-κB inhibitor-PDTC on Wnt signal pathway in mice postinfarction /HUANG Ying,MA Yitong, YANG Yining，LIU Fen,CHEN Bangdang, LI Xiaomei//Department of Coronary Artery Disease， The 1st Affiliated Hospital，Xinjiang Medical University，Urumqi，Xinjiang，830054，China 　　Corresponding author: MA Yi-tong, Email：myt-xj@163.com 　　Abstract：Objective:To study the effects of NF-κB inhibitor -PDTC on expression of protein related to Wnt signal pathway in mice post-infarction.Methods:Choose 3 months C57/BL mice to set up acute myocardial infarction animal model by ligation of left coronary artery .They were divided to AMI+PDTC group(36 cases, mice was treated with injected PDTC on dose of 120 mg/kg·d)and AMI group (36 cases, mice was treated with injected physiological saline with same volume).They were divided into 3 subgroups according to time postinfarction: 3d, 7d, 14d post infarction and detected expression of p65 related to NF-κB signal pathway by immunohistochemistry; and protein related to Wnt signal pathway: dvl-1 mRNA by RT-PCR, total (t)and phosphorylation (p) level of glucogen synthesis kinase(GSK)-3β，β-catenin，connexin 43 by Westernblot in left ventricular. Results: Expression of p65 decreased in AMI+PDTC group (P<0.05). Co