完整版双向电泳原理与流程.ppt

31 / GE Title or job number / 2/16/2020 pH 3-10 L and NL from Prof. Angelika G? rg, Technical University Munich Tissue Proteins from Mouse Liver linear (L) nonlinear (NL) 32 / GE Title or job number / 2/16/2020 The IPGphor ? Platform 33 / GE Title or job number / 2/16/2020 IPG 胶条 水化 34 / GE Title or job number / 2/16/2020 IPG strip rehydration 如果水化时加入样品, 此体积是加入样品后的终体积 35 / GE Title or job number / 2/16/2020 加样品到胶条槽 36 / GE Title or job number / 2/16/2020 泡涨盘 VS 聚焦盘 37 / GE Title or job number / 2/16/2020 干胶条的水化 ? 主动水化:胶条槽内进行,大分子蛋白更容易进入胶 条; 30 - 120V , 10 - 24Hrs ? 被动水化:专用水化盒中进行 NEW ! – 无需覆盖油

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