Current applications of high-resolution mass spectrometry in drug metabolism studies英文精品课件.pdfVIP

Anal Bioanal Chem (2012) 403:1221– 1231 DOI 10.1007/s00216-012-5807-z REVIEW Current applications of high-resolution mass spectrometry in drug metabolism studies Markus R. Meyer Hans H. Maurer Received: 20 December 2011 /Accepted: 28 January 2012 /Published online: 16 February 2012 # Springer-Verlag 2012 Abstract This paper reviews high-resolution mass spec- measurements in many fields of bioanalysis such as metabo- trometry (HRMS) approaches published in 2007–2011 for lite identification and structure elucidation, analytical toxicol- the elucidation of drug metabolism with a focus on new ogy, and doping control is notably increasing [1–6]. This is therapeutics, new drugs of abuse, and doping agents using also represented by the remarkable increase of publications time-of-flight, single-stage Orbitrap, ion trap Orbitrap, and listed in PubMed over a 15-year period using the search term other Fourier transform MS-based techniques. The present “high resolution mass spectrometry drug metabolism” review provides an overview of metabolite-generating sys- (Fig. 1.). This development from under ten publications listed tems and assays used, sample preparation techniques, ioni- in the mid 1990s to nearly 100 in 2010 is not that surprising zation and fragmentation techniques, as well as data mining because new acceptably priced devices have become available strategies and software tools which were used in the and HR mass spectrometry (HRMS) can have numerous reviewed papers. Furthermore, HRMS-specific topics such benefits in certain applications. As shown for glibenclamide as demand for a certain resolution or a specific mass accu- metabolites derived from in vitro incubation samples, the racy are discussed in detail and correspond