多色流式细胞FACS调补偿与圈门.ppt

Compensation, Controls, and Data Collection;Compensation Goal;Does Compensation Increase Data Spread?;Cytometer Settings Workflow;Compensation for Tandem Dyes ;Compensation Rules—Part 1;Compensation Rules—Part 2;BD CompBeads;Unstained Compensation Control Tube;Compensation QC;Compensation Discussion Points;Controls;Choose Appropriate Controls;Gating Controls;FMO Example;Comparison of Gating Controls;Data Collection;125,000 lymphocytes collected;Statistical Significance of Results;Data Collection Discussion