实验十食品中有机磷农药残留量测定气相色谱法.ppt

Evaluation only. Created with Aspose.Slides for .NET 4.0 Client Profile 17.1. Copyright 2004-2017Aspose Pty Ltd. Evaluation only. Created with Aspose.Slides for .NET 4.0 Client Profile 17.1. Copyright 2004-2017Aspose Pty Ltd. 实验十四、食品中有机磷农药残留量 的测定气相色谱法 标准依据: GB/T500920-2003食品中有机磷农药残留量 的测定 Evaluaton onr. ith Aspose slides for NET 4o client P Evaluation only. Created with Aspose Slides for NET 4.0 Client Profile 171 Copyright 2004 -2017 Aspose Pty Ltd  Evaluation only. Created with Aspose.Slides for .NET 4.0 Client Profile 17.1. Copyright 2004-2017Aspose Pty Ltd. Evaluation only. Created with Aspose.Slides for .NET 4.0 Client Profile 17.1. Copyright 2004-2017Aspose Pty Ltd. 目的与要求 1.掌握气相色谱仪的工作原理及使用方法。 2.学习食品中有机磷农药残留的气相色谱测定方法。 二、原理 食品中残留的有机磷农药经有机溶剂提取并经净化、 浓缩后,注入气相色谱仪, 后在载气携带 色谱柱中分离,由火焰光度检测器检测。当含有机 磷的试样在检测器中的富氢焰上燃烧时,以HPO碎 片的形式,放射出波长为526nm的特性光,这种光 经检测器的单色器(滤光片)将非特征光谱滤除后 由光电倍增管接收,产生电信号而被检出。试样的 峰面积或峰高与标准品的峰面积或峰髙进行比较定 Evaluaton onr. ith Aspose slides for NET 4o client P Evaluation only. Created with Aspose Slides for NET 4.0 Client Profile 171 Copyright 2004 -2017 Aspose Pty Ltd  Evaluation only. Created with Aspose.Slides for .NET 4.0 Client Profile 17.1. Copyright 2004-2017Aspose Pty Ltd. Evaluation only. Created with Aspose.Slides for .NET 4.0 Client Profile 17.1. Copyright 2004-2017Aspose Pty Ltd. 三、仪器与试剂 )仪器 气相色谱仪:附有火焰光度检测器(FPD) 2.电动振荡器 ■3.组织捣碎机 4.旋转蒸发仪 试剂 1.二氯甲烷; 2.丙酮; 3.无水硫酸钠:在700°C灼烧4h后备用 4.中性氧化铝:在550°℃C灼烧4h 5.硫酸钠溶液 6.有机磷农药标准贮备液: 7.有机磷农药标准使用液 Evaluaton onr. ith Aspose slides for NET 4o client P Evaluation only. Created with Aspose Slides for NET 4.0 Client Profile 171 Copyright 2004 -2017 Aspose Pty Ltd  Evaluation only. Created with Aspose.Slides for .NET 4.0 Client Profile 17.1. Copyright 2004-2017Aspose Pty Ltd. Evaluation only. Created with Aspose.Slides for .NET 4.0 Client Profile 17.1. Copyright 2004-2017Aspose Pty Ltd. 四、实验步骤 )样品处理 1.蔬菜:取适量蔬菜擦净,去掉不可食部分后称取蔬菜试样,将蔬 菜切碎混匀。称取10.0g混匀的试样,置于250mL具塞锥形瓶中 加3σg