液相色谱谱质谱联用.pptVIP

Agilent LC-MS/MS 6420  内容 1.LCMS/MS的基本原理 2.方法开发流程及优化 3.电离影响因素与溶液化学 4.注意事项 2017/1/5  1.L Octopole ion guide olic quadrupole Enhanced ion transmission a wider mass ran and spectral resolution ectral resolution Thin skimmer short High-pressure hexapole collision cell with Off-axis, high-energy dynode kimmer distance Reduced beam broadening transmission and increased Optimized MS/MS fragmentation without High gain with rapid polarity switching, long life, and low noise lon optics are opimized for ien transmission across a bread mass range  esi ion source lons Nebulizing gas eated nitrogen drying gas Eluent spray Entrance to dielectric capillary +镍 →套→  涂辰毛细管 层流声波液相色 谱洗脱液雾化器 进入质谱仪的离子 液滴喷雾 中性离子形成区 防止液滴扩散和去溶 剂的高温N2籟流气  Front-end ion optics Dielectric capillary Small diameter octopole ion guide (skimmer High frequency Rf octopole 2017/1/5  Quadrupole U-V cos 2a ft ions with U+Vcos 2 ft ions collide with the rods Scanning of measuring range U and v are varied U( DC voltage) 2017/1/ ac and dc voltages v(peak amplitude of a radio frequency)  Collision cell First quadrupole Quadrupole: ion focusing Hexapole Octopole: ion transmission 0.5 ml/min Entrance lens Exit lens Beam shaper am shap Third quadrupole  Rear quadrupole and detector Collision cell (hexapole Rear quadrupole Beam shaper Detector Exit lens 三2017/1/5  Pumping system 小 Pump Single 3-stage turbo pump 2tor10-210-3 105 torr torr torr 10