体外泛素化标准流程.docVIP

In vitro ubiquitination assay A. Total 15ul Reaction setup Rxn-mix (ul) E1-mix (ul) 5x Buffer 2.0 1 ATP (100mM) 0.5 - Ub (10mg/ml) 1.0 - UbcH7 (~10mg/ml) 0.5 - E1 - 0.5 E3 Sf1 (5ul) Sf2 (3ul) 5.0 - Compounds (5-10u