GB4789.32-2002 国家行业标准.pdfVIP

iCS07}10030 C 53 遏冒 中 华 人 民 共 和 国 国 家 标 准 GB/r4789.32-2002 食品卫生微生物学检验 大肠菌群的快速检测 Microbiologicalexaminationforfoodhygiene rapiddetectionofcoliform bacteria 2002一06一13发布 2002门0一01实施 中 华 人 民 共 和 匡 国家质量 监督检验检疫 总 辰 cs/T478932-2002 目 次 前言 引言 范围 ·······…… 规范性引用文件 术语和定义 ······································································································…… 1 原理 ············································。··································································…… 1 试剂和培养基 ········································································4··························…… 1 设备和器材 ····································································································…… 2 检验程序 ·········································································································…… 3 样品的制备 ····························································。··················。·············。········…… 3 9大肠菌群的MPN计数 ·················································································……二‘’二3 10大肠菌群的菌落计数···············。···························，··································……““·”‘”·‘4 附录A(规范性附录) 大肠菌群最可能数(MPV[)检索表 ················································…… 5 GB/z4789.32-2002 前 言 本标准规定了食品中大肠菌群的快速检验方法。本标准规定使用的培养基MUGaI肉汤中的4一甲 基伞形酮一各D一半乳糖昔与美国联邦法规40，第141节，21款(CodeofFederalRegulations40,Part 141.21)规定的检验大肠菌群所用的MI琼脂的主要成分(4一甲基伞形酮一p-D一半乳搪昔)是同一物质。 本标准关于大肠菌群的菌落计数，参考了ISO4832;1991(E)《微生物学— 大肠菌群菌落计数法 通则》(Microbiology-Generalguidancefortheenumerationofcoliforms-co