pMIR-Reporter使用说明[汇编].pdfVIP

pMIR-Reporter 编号 载体名称 北京华越洋VECT90294 pMIR-Reporter pMIR-Reporter 载体基本信息： 启动子: CMV,SP6,T7,Lac 复制子: pUC ori，SV40 ori 终止子: SV40 poly(A) signal 质粒分类: 哺乳细胞，信号通路报告载体 质粒大小: 6470bp 原核抗性: Amp 筛选标记: Puro 克隆菌株: DH5α 培养条件: 37℃，有氧 LB 表达宿主: 哺乳细胞 诱导方式: 无须诱导，瞬时表达 5测序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG 3测序引物: M13F：TGTAAAACGACGGCCAGT pMIR-Reporter 载体质粒图谱和多克隆位点信息： 1 / 6 pMIR-Reporter 载体简介： The pMIR- REPORT™ miRNA Expression Reporter Vector System consists of an experimental firefly luciferase reporter vector and an associated β-gal reporter control plasmid. By inserting predicted miRNA target sequences in the multiple cloning site, 2 / 6 pMIR-REPORT Luciferase miRNA Expression Reporter Vector can be used to conduct accurate, quantitative evaluations of miRNA function. It can also be used to evaluate siRNA target sites and to analyze the influence of 3 UTR sequences on gene expression. pMIR-REPORT Luciferase contains a firefly luciferase reporter gene under the control of a CMV promoter/termination system. The 3 UTR of the luciferase gene contains a multiple cloning site for insertion of predicted miRNA binding targets or other nucleotide sequences. By cloning a predicted miRNA target sequence into pMIR-REPORT, the luciferase reporter is subjected to regulation that mimics the miRNA target. pMIR-Reporter 载体序列： ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACA