《常用荧光探针小结》.pptVIP

常用荧光探针小结 * Exitation λmax: 495 nm; Emission λmax: 519 nm; Solvent pH:8.00 * Immunocytochemistry/Immunofluorescence-alpha Tubulin antibody [DM1A] (FITC) (ab64503) ICC/IF image of ab64503 stained human HeLa cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64503, 1μg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor? 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). * 二、荧光素酯 Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) is a cell permeable dye generally used in animal cell proliferation research. CFDA-SE enters cells by diffusion and is cleaved by intracellular esterase enzymes to form an amine-reactive product, CFSE. This product produces a detectable fluorescence and covalently binds to intracellular lysine residues and other amine sources. 酯酶 * 视频：The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation * 四甲基异硫氰酸罗达明，它是一种紫红色粉末，较稳定。其最大吸收光谱为550nm，最大发射光谱620nm，呈橙红色荧光，与FITC的黄绿色荧光对比清晰，与蛋白质结合方式同TITC。它可用于双标记示踪研究。 三、异硫氰酸罗丹明（TMRITC） * Detection of α-tubulin in A549 cells demonstrates use of rhodamine-labeled secondary antibody. Cells were probed with a mouse anti-α-tubulin primary antibody (0.4μg/mL) and Rhodamine-goat anti-mouse secondary antibody (2μg/mL). Nuclei were labeled with Hoechst Dye. Images were acquired by fluorescence microscopy. A. Fluorescence image shows a delicate network of α-tubulin (pseudo-colored green) located exclusively in the cytoplasm. B. Nuclear counterstain with Hoechst Dye (pseudo-colored blue) C. Merged image. * ?????????????????????????????????????????????????????????? AC41323-0010 RB200，也称丽丝胺罗丹明B 无定形褐红色粉末，不溶于水，易溶于酒精和丙酮，性质稳定，可长期保存，最大吸收光谱为570nm，呈明亮的橙色荧光，因与FITC的黄绿色有明显区别，故被广泛用于对比染色或用于两种不同颜色的荧光抗体的双重染色。 标记方法方法：取1g