硼替佐米对伯基特淋巴瘤Raji的体外治疗作用.docxVIP

硼替佐米对伯基特淋巴瘤 Raji的体外治疗作用 李靖1,闫明明2 1.河南省洛阳职业技术学院，河南洛阳， 471002 2.河南科技大学第一附属医院，河南洛阳， 471002 摘要：【目的】探究硼替佐米对伯基特淋巴瘤 Raji细胞体外治疗作用及其可能机制。【方 法】MTT实验检测硼替佐米对淋巴瘤 Raji细胞活力的影响； Western blotting实验和RT-PCR 实验检测硼替佐米对 Raji细胞中NF- k B p65表达和Caspase-3剪切的影响。【结果】 MTT 实验表明硼替佐米抑制 Raji细胞活力，并且其抑制作用呈剂量、时间依赖性（ P0.05）; Western blotting实验和RT-PCR实验显示硼替佐米显著抑制 Raji细胞NF- k B p65表达并且促 进caspase-3的剪切（P0.05）。【结论】 硼替佐米能够抑制 Raji细胞增殖，机制可能与抑 制NF- k B （ P65）激活和上调 cleaved-caspase-3表达有关。 【关键词】硼替佐米；淋巴瘤； NF- e B Thetherapeutic effect ofurkittbortezomib on The therapeutic effect of urkitt bortezomib on lymphoma Raji in vitro Li Jing1, Yan Ming-ming2 1:Luoyang Vocational College of Technology, Luoyang 471002,China 2:First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471002,China Abstract: Objective: To explore therapeutic effect of bortezomib on Burkitt lymphoma and its possible mechanism. Methods: The effect of bortezomib on human lymphoma Raji cells proliferation was determined by MTT assay. The expression of NF- k B p65 and Cleaved-caspase-3 protein were detected by Western blotting. NF- k B p65 and Cleaved-caspase-3 mRNA expression were detected by RT-PCR. Results: The data showed that bortezomib inhibited Raji cells proliferation and the inhibition rate were related in a dose-dependent manner and a time-dependent manner (P0.05). Western blotting data indicated that the expression of NF- k B p65 was decreased and the expression of Cleaved-caspase-3 was increased after bortezomib treatment in a dose dependent manner (P0.05). RT-PCR data demonstrated that the mRNA expression of NF- k B p65 was decreased and the mRNA expression of Cleaved-caspase-3 was increased after bortezomib treatment in a dose dependent manner (P0.05). Conclusions: Bortezomib can inhibit Raji cells proliferation may via downregulating the expression of NF- k B and upregulating the expression of Cleaved-caspase-3. Key words: Bortezomib; lymphoma; NF- k B 伯基特淋巴瘤（Burkitt lymphoma, BL），属于B淋巴细胞性的非霍奇金淋巴瘤（ NHL）, 常发于儿童和青少