小肽多拷贝基因表达载体的构建及其高效表达.docxVIP

中国生物化学与分子生物学报 2002年6月 !!!!：!竺!!! !b!!!!!』!!望堂』!j堡!!型!!窒塑!塑些璧堡坠!i!!!壁 !!!!!!竺!竺! A Series of pET-derived Vectors for Hi【gh·level Expression of the Gene of Multiple Copies Smaller Peptide in E．coli HU Xue-jun．ZHANG Zhi—chao，BAO Yong-ming，YANG Qing，AN LJ-jia。 (BioengineeringⅨmmⅧ，DahanⅢlmm ofTechnology，Dalian 116012，Oh／ha) Abstract A rapid method for constructing series of expression carrying multiple copies smaller pep- tide described．An artifieial gene of thyroosin al(28 amino acid residues)was used model be cloned inlo from pET32a(+)．A unique EcoTl4 I(CCAAGG)cloning site introduced into