瓜馥木总生物碱及3个阿朴菲类生物碱的抑菌活性研究.doc免费

瓜馥木总生物碱及3个阿朴菲类生物碱的抑菌活性研究 傅春燕1＊, 刘永辉2, 周中流3, 尹文清4, 陈凯玲4 (1. 邵阳医学高等专科学校药学系, 湖南邵阳 422000； 2. 湖南省新邵县红十字会医院, 湖南邵阳 422000； 3. 北京中医药大学中药学院，北京 100102； 4. , 广西桂林 541004 ) 摘 要：在室内用生长速率法测定了瓜馥木总生物碱及其3个阿朴菲类生物碱对甘蔗凤梨病菌、西瓜枯萎病菌、柑桔疮痂病菌、香蕉炭疽病菌、梨黑斑病菌和梨褐斑病菌的抑菌活性。结果表明：在供试质量浓度为10 mg/mL时，瓜馥木总生物碱对6种病原真菌具有很好的抑菌活性，它们的有效中浓度EC50分别为1.4535、1.3921、2.0200、1.9084、1.6413 和2.7041 mg/mL；从瓜馥木总生物碱分离得到的3个阿朴菲类生物碱对6种病菌均有较高的抑菌活性，其中1,2-亚甲二氧基-N-甲氧酰基-阿朴菲生物碱对6种病菌的EC50分别为0.1539、0.2334、0.1367、0.1735、0.4012和0.3168 mg/mL。 关键词：瓜馥木总生物碱；阿朴菲类生物碱；病原真菌；抑菌活性 Inhibitory activity of the total alkaloid from Fissistigma oldhamii (Hemsl.) Merr. and its three aporphine alkaloids against pathogenic fungi FU Chun-yan1＊, LIU Yong-hui2, ZHOU Zhong-liu 3, YIN Wen-qing4, CHEN Kai-ling4 (1. Department of Pharmacy, Shaoyang Medical College, Shaoyang 422000; 2. Red Cross Hospital at Xinshao County of Hunan Province, Shaoyang 422000; 3. School of Chinese Pharmacy Beijing University Abstract：The inhibitory activity of the total alkaloid from Fissistigma oldhamii (Hemsl.) Merr. and its three aporphine alkaloids against Thielaviopsis paradoxa (de Seynes) V. Hohnel, Fusarium oxysporum f.sp.Niveum, Sphaceloma fawcettii Jen kins, Glorosprium musarum (Berk ＆ Curt) Arx, Alternaria kikuchiana Takana and Mycosphaerella sentina (Fr.) was determined in laboratory using the growth rate method. The results showed the total alkaloid from Fissistigma oldhamii had a good inhibitory activity against the six pathogenic fungi at a concentration of 10 mg/mL, and the EC50 were 1.4535, 1.3921, 2.0200, 1.9084, 1.6413 and 2.7041mg/mL, respectively. The three aporphine alkaloids isolated from Fissistigma oldhamii had high inhibitory activity against the six pathogenic fungi. Among the three, the EC50 of Romucosine were 0.1539, 0.1735, 0.4012, 0.2334, 0.1367 and 0.3168 mg/mL. Key words：Total alkaloid of Fissistigma oldhamii; Aporphine alkaloids; Pathogenic fungi; Inhibitory activities 瓜馥木 (Fissistigma oldhamii (Hems1.) Merr.)，又名铁钻，是番荔枝科瓜馥木(Fissistigma Griff.)植物