决明子提取物对急性肝损伤保护作用的实验研究.docxVIP

决明子提取物对急性肝损伤保护作 用的实验研究 【关键词】提取物 摘要：目的观察决明子提取物对 CCI4所致 小鼠急性肝损伤的影响。方法 60只雄性昆 明小鼠随机分为6组：正常对照组、模型对 照组、阳性对照组、大、中、小剂量给药组。 大、中、小剂量给药组按，，g/kg灌胃决明 子提取物，阳性对照组按 g/kg灌胃联苯双 酯，正常对照组及模型对照组以等体积生理 盐水灌胃，共7d。第6天，模型对照组、 阳性对照组和给药组按 ml/kg背部皮下注 射CCI4,正常对照组背部皮下注射等体积生 理盐水。比较各组血清门冬氨酸氨基转移酶、 丙氨酸氨基转移酶，超氧化物歧化酶 (superoxide dismutase , SOD)丙二醛 (malondialdehyde , MDA)并观察肝组织形 态学变化。结果决明子提取物能显着降低 CCl4所致小鼠血清 AST、ALT含量的升高， 并能升高SOD勺活性，降低MDA^量；减轻 对肝细胞的病理性损害。结论决明子提取物 对CCI4所致的小鼠急性肝损伤具有一定的 保护作用。 关键词：决明子； 急性肝损伤； 保护作 用 Experimental Study on Protective Effect of SemenCassiae Extract Against Acute Liver Injury Abstract : ObjectiveTo observe the effect of Semen Cassiaeextract against carbon tetrachIoride induced actute Iiver injury in mice. MethodsSixty maIe mice were randomIy divided into normaI control group , model control group , bifendate pills control group , high-dosage group ， middle- dosage group and low- dosage group. The mice in the above groups were fed for 7 days. The drug groups were pretreated intragastrically with ,, g/kg extract , bifendate pills groups were pretreated intragastrically with g/kg bifendate. Control animals were given the same volumn physiological saline. On the 6th day of feeding, the mice except normal control group were given ml/kg carbon tetrachloride by subcutaneous injection. Twenty-four hours after the intoxication , the serum AST , ALT, SOD activities and MDAcontent were measured in each group above. ResultsSemen Cassiae extract significantly decreased carbon tetrachloride induced actue hepatic injury with increasing the activities of AST , ALT and the content of MDA , increased the activities of SOD in serum , the severity of liver injury was slight in the high dosage group in pathological examination. ConclusionIt is revealed that Semen Cassiae extract could prevent carbon tetrachloride from inducing acute liver injury. Key words : SemenCassiae extract ; Liver injury ; Protective effect 决明子Semen Cassiae属豆科植物决明子 成熟干燥种子，处方名为草决明、生决明。 决明子具有降血压、降血脂、导泻、抗菌、 抗血小板聚集等多种作用:1~3］。本实验