食管鳞癌受体基因启动子区甲基化状态.pptVIP

;并非所有暴露 个体均患病 ;;DNA 甲基化是在DNA甲基转移酶的作用下,以S-腺苷甲硫氨酸为甲基供体,将甲基基团转移到胞嘧啶第5位碳原子上,从而转变成5-甲基胞嘧啶（5-mC）。;冀医四院; 头颈部鳞癌、胃癌及胰腺癌细胞株中TβRⅠ基因甲基化与其表达降低有关。;研究目的;; 基因; Methylation analysis of TβRⅠand TβRⅡ in tumor tissue (T) and corresponding normal tissues (N). u: indicates the presence of unmethylated genes; m: indicates the presence of methylated genes. Cases 1: tumor-specific methylation; Case 2: the tumor is fully methylated, whereas the corresponding normal tissue has a very faint band demonstrating methylation; Case 3: both of tumor and corresponding normal tissue unmethylated.;Unmethylated;Frequency of TβRⅠ,Ⅱ gene methylation in ESCC and adjacent non-cancerous tissues; The relationship between TβRⅠ,Ⅱ gene methylation and clinical data in ESCC; Frequency of TβRⅠ,Ⅱ gene methylation in common in ESCC and adjacent non-cancerous tissue;The relationship between TβRⅠ,Ⅱ gene methylation in common and clinical dates in ESCC; 目前认为DNA甲基化主要在转录水平抑制基因的表达，TGF-β受体Ⅰ、Ⅱ基因的甲基化对其mRNA的表达是否有影响呢？; 基因; RT-PCR analysis of TβRⅠ ma:100bp DNA marker; 1,3,5,6: positive mRNA expression; 2,4: negative mRNA expression;The expression of TβRⅠ,Ⅱ mRNA in ESCC tumor and adjacent non-cancerous tissues;Gene; 本研究结果显示食管鳞癌组织中TβRⅡ的甲基化率高于癌旁组织，其mRNA的表达量也明显低于癌旁组织，但是发生甲基化的癌组织与未发生甲基化的癌组织相比，其mRNA表达虽然降低，但却无统计学差异。 ;部分肿瘤组织可能由于基因启动子区甲基化程度不足以抑制转录而导致了发生甲基化的组织其mRNA表达仍可为阳性。 ;结 论;Thanks for your attention!