生物化学教学课件：Group Meeting_ZHENG Yizhe_Detecting small quantity of biomolecules -071009.pptVIP

Detecting small quantity of biomolecules ZHENG Yizhe Outline 2 wide-used techniques ELISA PCR ImmunoPCR Chad Mirkin’s work Our proposal ELISA (Enzyme-Linked ImmunoSorbent Assay ) Antigens all substances that can be recognized by the adaptive immune system Antibodies are Y-shaped proteins that are found in blood or other bodily fluids of vertebrates, and are used by the immune system to identify and neutralize foreign objects Classification of ELISA Indirect ELISA Sandwich ELISA Competitive ELISA Indirect ELISA Indirect ELISA Sandwich ELISA Competitive ELISA PCR (Polymerase Chain Reaction) K