霉变奶酪对小白鼠肝肾功能指标的影响研究.pdfVIP

摘 要 本实验以小白鼠为实验对象，日粮中添加霉变的奶酪，饲养65d，测定有关肝肾 功能的血液生化指标，分析数据，以探讨该食品对小白鼠肝肾功能是否会产生影响。 实验方法：使奶酪室温条件下自然霉变15d，剪下其菌丝，美蓝染色，直接镜检， 对比霉菌的菌丝与孢子形态，进行初步的种类鉴定，之后将霉变奶酪冷冻保存，用于 饲喂小白鼠。选择健康清洁级昆明小白鼠84只，雌雄各半，雌雄分笼饲养，随机分组。 实验组每组12只，对照组12只，空白对照组12只。实验组饲喂霉变奶酪+普通全价鼠 粮，对照组饲喂普通全价鼠粮+正常奶酪，空白对照组饲喂普通全价鼠粮，自由采食。 实验组分为A组、B组、C组、D组、E组，分别饲喂25、35、45、55、65d后，进行处理， 对照组与空白对照组则在饲喂的65d进行处理。 实验结果：对比实验霉菌的形态特征，初步鉴定本实验中引起奶酪霉变的霉菌为 曲霉，且伴生有酵母菌；本试验中所用的霉变奶酪对小白鼠的肝脏功能未产生明显的 影响；本实验中的霉变奶酪对小白鼠血糖、血清蛋白及WBC 等指标无明显的影响；本 实验中的霉变奶酪使得小白鼠血清UR、Cr 水平显著下降。 关键词：蒙古族奶酪；霉变；肝肾功能 Studies on the Effects of Moldy Horod to the Indicators of the Function of Liver and Kidney Abstract In this experiment, the mice as experimental subjects, supplemented with moldy diet, fed 65d, determination the parameters of blood biochemical of liver and kidney function, analyzed the data, In order to reach the food will have effect on the function of liver and kidney in mice. The experimental methods were as follows: Made the horod naturally moldy 15 days under the room temperature, cut the mycelium, stain ed by methylene blue and direct ly detected under the microscope, contrasted its fungal hyphae and spore morphology, for preliminary species identification, after then cryopreservation of moldy horod for fed the mice. Choose 84 Kunming mice, healthy and clean, male of half, male and female were housed separately randomized. Each group, n = 12. The experimental group fed by moldy horod and normal full price of rat food, control group fed by normal full price of rat food and normal horod, control group was fed ordinary full price rat food, adlibitum. The experimental gro