12、国外大学研究所收集谷歌谷歌-ngs-pipeline的sequencing.pptVIP

* * * * * * * * * Effects of read number in ChIP-seq Hongkai Ji et al. Nature Biotechnology 26: 1293-1300. 2008 RNA-Seq Mortazavi et al. Nature Methods,5:621-628, 2008 After two rounds of poly(A) selection, RNA is fragmented to an average length of 200 nt by magnesium-catalyzed hydrolysis and then converted into cDNA by random priming. The cDNA is then converted into a molecular library for Illumina/Solexa 1G sequencing, and the resulting 25-bp reads are mapped onto the genome. Normalized transcript prevalence is calculated with an algorithm from the ERANGE package. (b) Primary data from mous