蛋白质与生物大分子的相互作用研究1-1.pptVIP

* ZEBRA mutants that fail to activate viral replication are defective at binding DNA in vitro.（K赖氨酸，R精氨酸）（+，表示加入非标记探针） A) Electrophoretic mobility shift assay (EMSA) comparing the DNA binding activity of Z(Y180E), Z(K188A), Z(K188R) and wt ZEBRA using four ZEBRA response elements. ZREs 1-3 are present in the origin of lytic replication (oriLyt), while ZIIIB is present in the bzlf1 promoter. The latter site has the highest affinity to ZEBRA among other known ZREs and was used as a positive control for binding. Probes were shifted using HKB5/B5 cell extracts expressing different ZEBRA proteins.