沉默干扰素诱导蛋白16对人主动脉外膜成纤维细胞凋亡的影响 肖燕.docxVIP

沉默干扰素诱导蛋白16对人主动脉外膜成纤维细胞凋亡的影响 肖燕 沉默干扰素诱导蛋白16对人主动脉外膜成纤维细胞凋亡的影响lowbar;肖燕 本文摘自内蒙古中医药 沉默干扰素诱导蛋白16对人主动脉外膜成纤维细胞 （1．贵州医科大学内科学教研室，贵州贵阳550004；2．贵州医科大学附属医院人民医院心内科，贵州贵阳550002） ［摘要］目的：探讨沉默干扰素诱导蛋白16（IFI16）表达对人主动脉外膜成纤维细胞（HAAFs ）凋亡的影响及 Control siＲNA转染HAAFs 分别IFI16基因沉默组（IFI16-其部分机制。方法：应用IFI16小干扰ＲAN（siＲNA）、 siＲNA组）、siＲNA组），阴性对照组（Control-未经干预的HAAFs 作为空白组；用流式细胞仪检测凋亡，应用West-ern blot 检测IFI16、EＲK1/2）蛋白表达。结果：与Con-细胞外调节蛋白激酶1/2（EＲK1/2）、磷酸化EＲK1/2（p-trol-siＲNA组或空白组相比，IFI16-siＲNA组的细胞凋亡率下降，p-EＲK1/2水平增伴随着IFI16蛋白表达减少，高，差异有统计学意义（P ＜0．05）。结论：沉默IFI16表达可减少HAAFs 细胞凋亡，其机制可能部分与EＲK信号通路有关。 ［关键词］干扰素诱导蛋白；血管外膜成纤维细胞；凋亡［［［2707（2021）05-0507-04中图分类号］Ｒ541文献标识码］A 文章编号］1000-DOI ：10．19367/j．cnki．1000-2707．2021．05．003 ＲeducingEffect of IFI 16Expression on Human Aortic Adventitial Fibroblasts Apoptosis XIAO Yan 1，WU Qiang 2 （1．Department of Internal Medicine ，Guizhou Medcial University ，Guiyang 550004，Guizhou ，China ；2．Department of Cardiology ， Guizhou Province People＇s Hospital Affiliated to Guizhou Medcial University ，Guiyang 550002，Guizhou ，China ） ［Abstract ］Objective ：To investigate the effect of silencing interferon-inducible protein 16（IFI16）expression on the apoptosis of human aortic adventitial fibroblasts （HAAFs ）．Methods ：The specific small interference ＲNAs（siＲNAs）of IFI 16and Control siＲNAwere transected into HAAFs in IFI16-siＲNAgroup ，negative control group and untreated blank group．Cell apoptosis was analyzed by flow cytometry．The protein levels of IFI16，extracellular signal-regulated kinase （EＲK1/2）and phospho-rylated extracellular signal-regulated kinase （p-EＲK1/2）were measured by Western Blot．Ｒesults： The protein expression levels of IFI16were decreased in the HAAFs ，the cell apoptosis was downregu-lated ，the protein expression levels of p-EＲK1/2were increased ，differences were statistic significant （P ＜0．05）．Conclusion ：Inhibition of IFI16expression can downregulate HAAFs cell apoptosis ，which may be related to the EＲKsignaling pathway．