不同地理种群小菜蛾的遗传多样性分析 王少丽.docxVIP

不同地理种群小菜蛾的遗传多样性分析 王少丽 不同地理种群小菜蛾的遗传多样性分析lowbar;王少丽 第22卷第10期2002年10月 生　　态　　学　　报V ol. 22, No. 10Oc t. , 2002 不同地理种群小菜蛾的遗传多样性分析 王少丽, 盛承发, 乔传令* (中国科学院动物研究所农业虫鼠害综合治理研究国家重点实验室, 北京　100080) 摘要:采用水平切片淀粉凝胶电泳方法, 分析了北京、河北、云南和武汉4个不同地理种群的小菜蛾的等位酶, 得到了3个酶系统(甘油醛磷酸脱氢酶(GP D ) 、苹果酸酶(ME ) 和苹果酸脱氢酶(MD H -1, MD H -2和MD H -3) ) 5个基因位点的资料。用Bios ys 2. 0软件计算了不同地理种群的遗传变异指标(N 、A 、P 、H o 和H e ) , 结果表明小菜蛾各种群内的基因多样性大于各种群间的基因多样性(约15倍) , 武汉种群小菜蛾的遗传多样性最大。并计算了遗传距离D 和相似性系数, 并由此得出聚类图, 分析了小菜蛾不同地理种群间的遗传关系。关键词:等位酶; 小菜蛾; 淀粉凝胶电泳; Biosys 2. 0; 遗传多样性 :Plutellidae ) Genetic Variability of Plutella xylostella (Lepidoptera Population From Different Geographies W ANG Shao-Li, SHEN G Cheng -Fa, Q IAO Chua n-Ling 　(State Ecolog ica Sinica , 2002, 22(10):1718～1723. Key Laboratory of Integ rated Manag ement of Pest Insects and Rodents , Institute of Zoology , Ch ines e Academy of Scienc es , Beijing 10080, China ). Acta Abstract :The a na ly sis of a llo zy mes ha s many specific adva ntag es in ana ly zing g enetic v ariabilities and di-v er sities , so it has been widely used in the po pulatio n differ entiation , div er sity and differ entiatio n o f species . Some pr ev io us studies hav e been documented tha t allo zy me analy sis , g ene flo w a nd evo lutio n in-ter -population ar e clo sely rela ted. Diamo ndback mo th (DBM , Plutella x ylostella L. ) is a cosmo po litan in-sec t pest, which mainly feeds o n cr ucife rous v eg etables. Widespread use o f insecticides ha s led to the selec-tio n of r esistance in DBM . T he pr esent w or k w as underta ken to study the g enetic v ariability of DBM fro m differ ent g eog ra phies . Four po pula tio ns o f DBM w ere co llected fro m Beijing , Hebei, Yunna n a nd W uhan in China in 1997. They we re r ea red in the la bo ra tor y until the 4th instar , and then deep-fr ozen for further analy sis. T he al-lo zymes w ere identified in sing le individua l homo ge nates analyzed by star ch electro pho resis with