非洲猪瘟诊断血清学技术.pptxVIP

African swine fever diagnostics: serological techniques 非洲猪瘟诊断 血清学技术,David Williams大卫·威廉姆斯Australian Animal Health Laboratory澳大利亚动物健康实验室Laboratory diagnostic algorithm for ASF ASF的实验室诊断算法Agent detection代理检测Serology血清Appropriate sample1适当的样品1Serum血清PCR (King, UPL, Zsak etc)Isolation(Primary cells– PBM, PAM)Ag ELISA(In-house or INGENASA)PosPosELISA(In-house, commercial)PosGene targets: B646L (P72), B602L, I73R- I329L IGRPosConventional PCR2(genotyping)IFA/IPX or immunoblot3常规PCR2（基因分型）PosSequencing2测序EDTA blood, spleen, lymph nodes, tonsils, kidneys EDTA血，脾，淋巴结，扁桃体，肾脏At start of outb