中药活性成分对蛋白质的构象影响及其机理研究-分析化学专业毕业论文.pdf

Abstract Traditional Chinese medicine haS played an irreplaceable role in modem healthcare system．Recently， modemmedicine，chemistry and biology researchers are focusing on active ingredients ofChinese traditional medicine，as well as mechanisms of these active ingredients in the treatment of diseases．Proteins are the specific features oflife activity implementer，most drug molecules change the character ofspecifiC functional protein by interacting with the specific functional protein and thus play its due role． In thiS article,we selected the most numerous protein of the circulatory system，the human serum albumin(HSA)，as well as pepsin，one ofthe main digestive en刁mes in the digestive system，as our research subjects．Furthermore，we also select cyclovirobuxine D，helicid and aurantio。obtusfil from the three categories of the active ingredients which are extracted from traditional Chinese medicine respectively，as another research subjects．We use variety of methods，including steady-state fluorescence，synchronous fluorescence(SFS)，time．resolved fluorescence lifetime，Ultraviolet·Visble(UV‘vis)，Fourier transform infrared spectroscopy(FT-双)，circulardichroism(CD)，MolecularModeling，to studyanddescribethe t3q)e， character and some important parameters of the interaction between cyclovirobuxine D，helicid，aurantio。 obtusinmadHSAorpepsin，besides，we also studiedthedifferenceand similaritiesoftheinteractionbetween aurantio-obtusin andHSAor pepsin． Results and Conclusion：Fluorescence quenching data demonstrate that all of cyclovirobuxine D，helicid and aurantio．obtusin Can quench the fluorescence ofliSA or pepsin，which means that all ofcyclovirobuxine D，helicidand aurantio．obtusin have interacted with HSA or pepsin．S3mchronous fluorescence data indicats thatcyclovirobuxineD，helicidandaurantio-obtusillquenched也efluorescenceofproteinby interactingwith t