成年斑胸草雀在体HVC-RA突触传递电生理特性.pdfVIP

232 Acta Physiologica Sinica , June 25, 2006, 58 (3): 232-236 Research Paper The electrophysiological properties of HVC-RA synaptic transmission in the adult zebra finch in vivo MENG Wei, WANG Xiao-Dong, XIAO Peng, LI Dong-Feng* College of Life Sciences, South China Normal University, Guangzhou 510631, China Abstract: The synaptic connection from high vocal center (HVC) to robust nucleus of the arcopallium (RA) is a pivotal part of vocal motor pathway in songbirds. Electrophysiological properties of HVC-RA synaptic transmission in adult male zebra finch (Taeniopygia guttata ) in vivo was investigated by using field potential recording method. Following electrical stimulation of HVC, the evoked field potentials recorded in RA were feeble. The results showed that the remarkable paired-pulse facilitation was induced by paired-pulse stimulation at HVC-RA synapses. The results also showed that the evoked field potentials were significantly decreased after a conditioning tetanic stimulation and finally recovered gradually within 15 min, which indicates tetanic stimulation-induced transient depression is present at HVC-RA synaptic transmission. These results suggest that properties of synaptic transmission in this pathway might play a role in controlling song production. Key words: HVC-RA synaptic transmission; electrophysiological properties; zebra finch (Taeniopygia guttata) 成年斑胸草雀在体HVC-RA 突触传递的电生理特性 孟 玮，王晓东，肖 鹏，李东风* 华南师范大学生命科学学院，广州 510631 摘 要：鸣禽高级发声中枢(high vocal center ，HVC)至弓状皮质栎核(robust nucleus of the arcopallium ，RA) 的突触传递是鸣唱 运动通路中的关键部分。本文运用在体场电位电生理记录的方法，研究了成年雄性斑胸草雀(Taeniopygia guttata) HVC-RA 突 触的电生理特性。实验结果显示，刺激HVC ，在RA 内所记录到的诱发场电位幅度较小。配对脉冲检测发现，HVC-RA 突 触传递具有明显的配对脉冲易化特性。当以强直刺激作用于HVC ，RA 内诱发场电位随即显著减小，并在15 min 内逐渐恢 复，表明HVC-RA 突触传递在强直刺激过后出现了短时抑制。该通路的突触传递特性可能与其在发声控制中的作用有关。以 上的