植酸酶基因在pIAβ8和pGAPZαA两种载体中的表达及酶学特性分析.pdfVIP

河南农业大学硕士论文 Summery Our objectives were to determine the biochemical characters of phytase from A． ficuum，tO compare the ability of phytase gene expression in E．coli by pIAp8 and pGAPZo．A vectors，and to choose theoptimum vector for further researches． A．ficuum was incubated with solid medium．Phytase WaS extracted and purified by salting-out process，dialysis，laminar analysis．The purified phytase was used for thermostabilityandpH determination．The optimumpHpoints ofphytasewere 2．5 and 5．5．and o