第3章基因工程的工具酶.pptxVIP

;Enzymes Used for Gene Engineering;1. Restriction Endonucleases;Major Classes of Restriction Endonucleases;Three Types of Restriction Endonucleases;;Some of the type II restriction enzymes;Restriction fragments with either blunt or sticky ends;Cleavage patterns of some common restriction endonucleases;Different restriction enzymes produce fragments of different lengths;Comparison of results from partial and complete digests;Restriction mapping;Isoschizomer: e.g. Hind III and Hsu I Xma I and Sma I Msp I and Hpa I Isocaudamer: e.