血红蛋白电泳的意义与判断.pptx

ADULT AND NEWBORN Hemoglobin screening with capillarys;目的(mùdì);血红蛋白(xuèhóng dànbái)的结构;三维结构(jiégòu);正常(zhèngcháng)结果;血红蛋白(xuèhóng dànbái)遗传病类型(HEMOGLOBINOPATHIES) ?;血红蛋白(xuèhóng dànbái)遗传病;异常血红蛋白(xuèhóng dànbái)类型;血红蛋白疾病(jíbìng)的区域分布图;;Alpha 地中海贫血(pínxuè);其他特征;基因型;镰刀(liándāo)型红细胞血症;C型血红蛋白(xuèhóng dànbái)分子病;D型血红蛋白(xuèhóng dànbái)分子病;E型血红蛋白(xuèhóng dànbái)分子病;下列情况下,需要进行(jìnxíng)血红蛋白检测;血红蛋白(xuèhóng dànbái)检测的几种方法;凝胶电泳结果(jiē guǒ)识别表 ;在pH梯度的凝胶片上, 根据蛋白不同的等电点,对蛋白进行分离 Hb电泳位置取决于缓冲液PH值 蛋白电泳至其等电点位置后,停止(tíngzhǐ)泳动 At pI=pH, Hb does not have a net charge and stops migrating 电泳分离效果好, 但时间长.需要经验丰富才能获得良好结果及进行结果判断;;;毛细管电泳(diàn yǒnɡ)法原理 ;此时,电渗力(EOF)远远强于电场力. 因此(yīncǐ),蛋白被动从正极向负极端移动.;Capillarys/Minicap血红蛋白(xuèhóng dànbái)电泳;Capillarys/Minicap血红蛋白(xuèhóng dànbái)检测标本要求;Capillarys Minicap 血红蛋白(xuèhóng dànbái)条带识别表;Capillarys/Minicap区带识别(shíbié)表;正常(zhèngcháng)Hb A2 质控品;Hb AFSC质控品, 可用来作为(zuòwéi)参照曲线,便于异常结果的判断;Hbs A, F,A2 检测(jiǎn cè)结果可通过颜色进行区分 ;主要异常(yìcháng)血红蛋白变异体;;Capillarys/Minicap血红蛋白试剂(shìjì)优势:;异常结果(jiē guǒ)分析;Case 2:31 Y Female, 血红蛋白(xuèhóng dànbái) 12.8, 红细胞压积 37.4 (I);31 Y Female, Hgb 12.8, Hematocrit 37.4 (II);Hb A 92.5% Hb F 2.6 Hb A2 4.9%;Hb H 4% Hb A 94.7% Hb A2 1.3%;Hb H and Bart’s - Alpha-thalassemia;Case 1: 13 Y Male, Hgb 8.1, Hct 24.0 (I);Case 1:13 Y Male, Hgb 8.1, Hematocrit 24.0 (II);Case 2:65 Y Male, Hgb 9.4, Hematocrit 27.3 (I);;21Y, pregnant woman,Hgb 13.1, hematocrit 38.6;Baby 2 months old,father from Martinique, mother from Central Africa, Hgb 11, hematocrit 29.8;Diabetic patient, 54 Y, Hgb 14.5, hematocrit 43.5, MCV 88 ;Hb A 51.8% Hb A2 2.9% Hb C 35.9%;Adopted child, 4 Y, originated from Haiti, Hgb 10.9, Hematocrit 31.3, MCV 76.9;Diabetic patient, 73 Y, Hgb 10.2, hematocrit 30.8, MCV 87.3;Hb A 90.3% Hb F 3.8% Hb A2 5.9%;Hb Lepore on Minicap/Capillarys;Hb Lepore;a47 Asp→His;Hb O-Arab;DIAGNOSTIC FLOW-CHART;A NEW FAST HEMOGLOBIN ELECTROPHORESIS METHOD FOR DRIED BLOOD SPOTS ON SEBIA CAPILLARYS? AUTOMATED SYSTEM;现