第三章细胞生物学研究方法.pptx

细胞生物学 Cell Biology 杭州师范大学 陈建明;第三章 细胞生物学研究方法 Study Methods of Cell Biology;3;Experimental organisms are valuable for the study of cell and developmental biology;培养的哺乳动物细胞—模式生物; Tissue culture Advantages of working with cultured cells over intact organism ● More homogeneous than cells in tissues ● Can control experimental conditions ● Can isolate single cells to grow into a colony of genetically homogeneous clone cells Disadvantages of tissue culture ● Does not always mimic in vivo conditions in terms of gene expression ● Chromosome abnormalities ● Cells are no